Summary
Type: SPE
Application #: 301260
Catalog#: 730180G - SPE Cartridges, Chromabond, NH2, 6ml, Glass, 500mg 30/pk
Detection:
Conditions: Column conditioning:
10 ml methylene chloride, 10 ml methanol, then 10 ml dist. water - 2-propanol
(9:1, v/v)
Sample application:
Aspirate 500 ml pretreated water sample through the column (about 5 ml/min)
Column washing:
2 ml dist. water - 2-propanol (9:1, v/v), then dry column (about 20 min, vacuum)
Elution:
elute with 4 x1 ml methylene chloride (let percolate first 1 ml into the
column packing without vacuum, then apply light vacuum), if necessary evaporate
in a stream of nitrogen and fill up with a suitable solvent
Authors: SPE department
Source: Macherey-Nagel